553327
WT1
Größe
£652.00
- SKU:
- 553327
- Reinheit:
- Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant protein fragment corresponding to aa1-181 from human WT1, expressed in E. coli.
- Klon:
- 7F9E8
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Weitere Details:
- This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminal and a proline/glutamine-rich DNA-binding domain at the N-terminal. It has an essential role in the normal development of the urogenital system and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat and that this process is tissue-restricted and developmentally regulated. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Flow Cytometry: 1:200-1:400 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Immunocytochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice