Ago, pan (Eukaryotic translation initiation factor 2C, 2, Protein argonaute-2, Argonaute 2, Protein slicer, Argonaute2, PAZ Piwi domain protein, Eukaryotic translation initiation factor 2C, Argonaute2, Argonaute4, Ago4, Protein argonaute-4)

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£607.00

SKU:: 551135
Reinheit:: Purified by Protein G affinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Ago, fused to His-Tag
Klon:: 2A8
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Argonaute (Ago) proteins are a family of proteins of ~95kD that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detects Ago on 10ug of HeLa cell lysate. Optimal dilutions to be determined by the researcher. Molecular Weight: ~95, 70kD (observed) Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice