551135-FITC
Ago, pan (Eukaryotic translation initiation factor 2C, 2, Protein argonaute-2, Argonaute 2, Protein slicer, Argonaute2, PAZ Piwi domain protein, Eukaryotic translation initiation factor 2C, Argonaute2, Argonaute4, Ago4, Protein argonaute-4) (FITC)
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£895.00
- SKU:
- 551135-FITC
- Reinheit:
- Purified by Protein G affinity chromatography
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Ago, fused to His-Tag
- Klon:
- 2A8
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
- Weitere Details:
- Argonaute (Ago) proteins are a family of proteins of ~95kD that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Molecular Weight: ~95, 70kD (observed) Positive Control: HeLa cell lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody
- Versandbedingungen:
- Blue Ice