B7-1, Recombinant, Woodchuck, aa30-237, Fc-Tag, Avi-Tag (Biotin)

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£1,015.00

SKU:: 544162
Molekulargewicht:: 52
Reinheit:: ≥90%
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol. Labeled with Biotin.
Weitere Details:: Source: Recombinant protein corresponding to aa30-237 from woodchuck B7-1, fused to Fc region of Human IgG1 and with a C-terminal Avi-Tag, expressed in a HEK293 cells. Molecular Weight: ~52kD Assay Conditions: Reaction done similar to human CTLA4:B7-1[biotin] BioAssay™ kit protocol. Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.