Luciferase

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£371.00

SKU:: 538623
Reinheit:: Purified by immunoaffinity chromatography from ascites.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Luciferase.
Klon:: 6B8
Format:: Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. Applications: Suitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Immunohistochemistry (paraffin): 1:100-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.