His-Tag (Agarose)

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£502.00

SKU:: 537212
Anwendung:: IP
Reinheit:: Purified by immunoaffinity chromatography from ascites.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to His-Tag.
Klon:: 5C3
Format:: Supplied as a 50% gel slurry suspended in PBS, pH 7.4, 0.02% sodium azide. Lableled with Agarose.
Weitere Details:: A Polyhistidine-Tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N-terminal or C-terminal of the protein. It is also known as hexa Histidine-Tag, 6xHis-Tag, and by the trademarked name His-Tag. Polyhistidine-tags are often used for affinity purification of Polyhistidine-Tag recombinant proteins expressed in E.coli and other prokaryotic expression systems. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. It is recommended to wash the agarose beads with appropriate buffer prior to use to remove sodium azide. Note: We do not recommended adding glycerol as a cryopreservative.