KMT5A, Recombinant, Human, aa195-352, His-Tag (N-lysine Methyltransferase KMT5A Isoform 1)

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£384.00

SKU:: 506189
Molekulargewicht:: 18.91651828
Reinheit:: >85% (SDS-PAGE)
Lagerbedingungen:: -20°C/-70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 1mM EDTA, 50% glycerol.
Weitere Details:: KMT5A, also known as N-lysine methyltransferase KMT5A isoform 1, is an enzyme which catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1). It is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. It acts as a barrier to prevent cellular senescence through chromatin-mediated regulation of senescence-associated metabolic remodeling. It mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. The loss of KMT5A concurrently stimulate nucleolar function and retinoblastoma protein-mediated mitochondrial metabolism. Description: Recombinant protein corresponding to aa195-352 of human KMT5A, fused to His-tag at C-terminus, expressed in Baculovirus. Molecular Weight: ~18.9kD (165aa), ~18-28kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (LAL) AA Sequence: MKAELQSEER KRIDELIESG KEEGMKIDLI DGKGRGVIAT KQFSRGDFVV EYHGDLIEIT DAKKREALYA QDPSTGCYMY YFQYLSKTYC VDATRETNRL GRLINHSKCG NCQTKLHDID GVPHLILIAS RDIAAGEELL YDYGDRSKAS IEAHPWLKHH HHHHH Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice