502733
Secreted Frizzled Related Protein 4 (SFRP4) [KO Validated]
Größe
£544.00
- SKU:
- 502733
- Reinheit:
- Purified by Protein G affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met265-Val346 of human SFRP4, expressed in E. coli.
- Klon:
- C1
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Weitere Details:
- Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. Sequence analysis predicted that the 346aa SFRP4 protein contains an N-terminal signal peptide, no transmembrane domain, and a hydrophilic C terminus. In situ hybridization analysis demonstrated exclusive expression in stromal and myometrial cells, particularly in endometrium and breast. Upregulated expression was detected in endometrial and breast carcinomas. Applications: Suitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-5ug/ml Immunohistochemistry (frozen): 5-30ug/ml Immunohistochemistry (Paraffin): 5-30ug/ml Immunocytochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice