UBA3 (NEDD8-Activating Enzyme E1 Catalytic Subunit, NEDD8-Activating Enzyme E1C, Ubiquitin-Activating Enzyme E1C, Ubiquitin-Like Modifier-Activating Enzyme 3, Ubiquitin-Activating Enzyme 3, UBE1C)

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£394.00

SKU:: 407264
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa234-463 of human UBA3.
Format:: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.