DNA Ligase, Recombinant, Desulfotalea Psychrophila, aa340-679, His-tag (ligA)

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£518.00

SKU:: 405911
Molekulargewicht:: 40.4
Reinheit:: ~85% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Weitere Details:: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. Source: Recombinant protein corresponding to aa340-679 from Desulfotalea psychrophila DNA Ligase, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~40.4kD AA Sequence: RPQTSRLGRVNPVAEVEPTRLSGALIQRVTAHHYAMVRDRGIGPGARIRMSRSGEVIPKIEEVVKPVAAAQLDIPAHCPSCGSDLAWDGDYLFCLNNMSCPAQITNSMEHFFKILGNVDGFGPSSVQRLYEGGLVDLPALYAMESADFEQVGFGPKQATNMVEQLNRSREELIEDWRFLAAFGVHRLGMGNCEKLLRYVRLEDVFNLSEEQIVAEIKGFQEKTAHSICAGLAGIKDLFFQLFALGFKLEPTPLLAEGGQGEKSPISGKTIVFTGAMLAASRGDMEKQAKALGATVGKSITGKTDMLVTGQRVGATKMAKAETLGIAILSEEAYRQLVSLD Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.