404461
Splicing Factor 1 (SF1)
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£479.00
- SKU:
- 404461
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa48-97 from human SF1.
- Format:
- Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000. Immunohistochemistry (paraffin): 1:100-1:300. Immunofluorescence: 1:200-1:1000. ELISA: 1:20,000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice