CD335 (NKp46, Natural Cytotoxicity Triggering Receptor 1, NCR1) (APC)

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£832.00

SKU:: 397017
Anwendung:: Flow Cytometry
Reinheit:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein corresponding to extracellular region of porcine CD335.
Klon:: VIV-KM1
Format:: Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml dH2O. Labeled with Allophycocyanin (APC).
Weitere Details:: CD335, also known as NKp46 and natural cytotoxicity triggering receptor 1 (NCR1), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor and is involved in the control of viral infection and tumor development. Until recently little has been known about porcine and veterinary NK cells. CD335 is expressed by human natural killer cells and the development of monoclonal antibodies to bovine CD335 and sheep CD335 have enabled researchers to identify and better understand ruminant NK cells. This is the first monoclonal developed to specifically identify porcine CD335 and provides a reagent to facilitate a better understanding of the porcine immune system and aid in the understanding of the role of NK cells in host pathogen defense. Studies have shown that, within the porcine, CD335 is not universally expressed by all NK cells and that expression of this marker on NK cells may be influenced by cytokine production. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Versandbedingungen:: Blue Ice