ATF2, phosphorylated (Thr52) (CREB2, CREBP1, Activating Transcription Factor 2, Cyclic AMP-dependent Transcription Factor ATF-2, Cyclic AMP-Responsive Element- Binding Protein 2)

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£696.00

SKU:: 396967
Anwendung:: WB
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr52 of human ATF2, conjugated to KLH.
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 50% glycerol.
Weitere Details:: The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr-52 by PKCε regulates this translocation (Lau et al., 2012). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.