Il6, Recombinant, Rat, aa25-211, His-Tag (Interleukin 6, Ifnb2, ILg6)

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£371.00

SKU:: 396890
Molekulargewicht:: 22.8
Reinheit:: ≥95% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Weitere Details:: Il6, also known as interleukin 6, is an alpha-helical phosphorylated and glycosylated cytokine. This protein is important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism and cancer progression. Also, it can function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells. Recombinant rat Il6, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa25-211 of rat Il6, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~22.8kD (196aa), ~18-28kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: ADPFPTSQVR RGDFTEDTTH NRPVYTTSQV GGLITYVLRE ILEMRKELCN GNSDCMNSDDALSENNLKLP EIQRNDGCFQ TGYNQEICLL KICSGLLEFR FYLEFVKNNL QDNKKDKARVIQSNTETLVH IFKQEIKDSY KIVLPTPTSN ALLMEKLESQ KEWLRTKTIQ LILKALEEFL KVTMRSTRQT HHHHHH Biological Activity: Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED50 for this effects is less or equal to 0.1ng/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice