IFNG, Recombinant, Human, aa24-161, His-Tag (Interferon Gamma, IFG, IFI, Interferon Gamma)

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£371.00

SKU:: 396882
Molekulargewicht:: 18.4
Reinheit:: ≥90% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 20% glycerol.
Weitere Details:: IFNG, also known as Interferon gamma, is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Nature human interferon-gamma is composed of 143aa residues without cysteine residues and is glycosylated. Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Recombinant human IFNG protein, fused to His-Tag at N-terminal, expressed in E.coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa24-161 of human IFNG protein, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~18.4kD (159aa), Confirmed by MALDI-TOF Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG Biological Activity: Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is less or equal to 5ng/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice