Histone H2B type 1-B, acetylated (K20) (Histone H2B.1, Histone H2B.f, H2B/f, HIST1H2BB, H2BFF)

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£422.00

SKU:: 396517
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human Histone H2B type1-B.
Klon:: 17L25
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:30-1:200 Western Blot: 1:5000-1:10,000 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.