ERp57 (ERp60, ERp61, Grp57, Grp58, P58, PDIA3, PI PLC, 58kD Glucose Regulated Protein, 58kD Glucose-Regulated Protein, 58kD Microsomal Protein, Disulfide Isomerase ER 60, Disulfide Isomerase ER-60, Endoplasmic Reticulum Resident Protein 57, Endoplasmic Reticulum Resident Protein 60, ER p57, ER Protein 57, ER Protein 60, ERp 57, ERp57, Glucose Regulated Protein 58kD, GRP 57, GRP 58, GRP57, HsT17083, p58, PDIA 3, PDIA3, PDIA3 HUMAN, Phospholipase C Alpha, PI PLC, Protein Disulfide Isomerase A3, Protein Disulfide Isomerase Family A Member 3, Protein Disulfide-Isomerase A3)

Größe

£524.00

SKU:: 384891
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human ERp57 (Grp58).
Klon:: 4F9
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Weitere Details:: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7). Applications: Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry/Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice