PRMT4/CARM1 (Carm1, CARM1 HUMAN, Coactivator Associated Arginine Methyltransferase 1, Coactivator-Associated Arginine Methyltransferase 1, Histone Arginine Methyltransferase CARM 1, Histone Arginine Methyltransferase CARM1, Histone-Arginine Methyltransferase CARM1, PRMT 4, PRMT4, Protein Arginine Methyltransferase, Protein Arginine N Methyltransferase 4, Protein Arginine N-Methyltransferase 4)

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£633.00

SKU:: 381690
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to PRMT4/CARM1.
Klon:: 17J115
Format:: Supplied as a liquid in 0.1M Tris-HCl, pH 7.4, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 30% glycerol.
Weitere Details:: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs. Applications: Suitable for use in Western Blot, Immnoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:200-1:500 Immunoprecipitation: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice