DUSP23, Recombinant, Human, aa1-150, His-Tag (Dual Specificity Phosphatase 23, Low Molecular Mass Dual Specificity Phosphatase 3, LDP-3, MOSP, VHZ)

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£371.00

SKU:: 377790
Molekulargewicht:: 18.8
Reinheit:: ≥90% (SDS-PAGE)
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 100mM sodium chloride, 40% glycerol.
Weitere Details:: DUSP23, also known as low molecular mass dual specificity phosphatase 3 (LDP-3), belongs to the protein-tyrosine phosphatase family. This protein is a protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. This protein able to enhance activation of JNK and p38(MAPK14). Source: Recombinant protein corresponding to aa1-150 from human DUSP23, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~18.8kD (170aa), confirmed by MALDI-TOF Biological Activity: Specific activity is >200units/mg, and is defined as the amount of enzyme that hydrolyze 1nmole of p-nitrophenyl phosphate (pNPP)/minute at pH 7.5 at 37°C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGVQPPNFSW VLPGRLAGLA LPRLPAHYQF LLDLGVRHLV SLTERGPPHS DSCPGLTLHR LRIPDFCPPA PDQIDRFVQI VDEANARGEA VGVHCALGFG RTGTMLACYL VKERGLAAGD AIAEIRRLRP GSIETYEQEK AVFQFYQRTK Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Versandbedingungen:: Blue Ice