PRMT8, Recombinant, Human, aa61-394, His-FLAG-Tag (Protein Arginine N-methyltransferase 8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4)

Größe

£1,002.00

SKU:: 377021
Molekulargewicht:: 40.8
Reinheit:: ~92% (SDS-PAGE)
Lagerbedingungen:: -70°C
Hersteller:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 80ug/ml FLAG peptide, 3mM DTT, 20% glycerol.
Weitere Details:: Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS. Source: Recombinant protein corresponding to aa61-394 from human PRMT8, fused to His-FLAG-Tag at N-terminal, expressed in Sf9 cells using a Baculovirus expression system. Molecular Weight: ~40.8kD Specific Activity: ~2.22pmol/min/ug Assay Conditions: 50ul reaction mix (20mM phosphate buffer pH 7.4, 20uM S-adenosyl methionine, and 1-5ng methyltransferase PRMT8) added to the wells coated with the substrate. Incubate at RT for 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally,add HRP chemiluminescent substrates and read luminesence. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.