c-Jun, NT (Transcription Factor AP-1, Activator Protein 1, AP1, Proto-oncogene c-Jun, V-jun Avian Sarcoma Virus 17 Oncogene Homolog, p39, JUN)

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£524.00

SKU:: 376930
Reinheit:: Purified
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: GST-protein corresponding to the N-terminal sequence of human c-Jun.
Klon:: 60A8
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Weitere Details:: c-Jun is a member of the Jun Family composed of c-Jun, JunB and JunD, and is a component of the transcription factor AP-1. AP-1 is composed dimers of Fos, Jun and ATF family members, and binds and activates transcription at TRE/AP-1 elements (Reviewed in 1). Extracellular signals including growth factors, chemokines and extracellualar stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and the context of the cell (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 Immunohistochemistry (Frozen): 1:400 Immunofluorescence (IF-IC): 1:50 ChIP: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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