369669
XRN1 (5'-3'Exoribonuclease 1, SEP1, Exoribonuclease I, Strand-exchange protein 1 homolog)
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£626.00
- SKU:
- 369669
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- XRN1 (Tyr1394~Glu1706)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- XRN1 is an exoribonuclease enzyme. This enzyme is involves in 5’ to 3’ RNA degradation. The deduced 1694aa protein has a calculated molecular mass of 190kD. It shares 92% homology with mouse Sep1, which the authors called Dhm1, over the N-terminal and central portions of the molecule, but only 74% homology at the C terminal. Northern blot analysis detected a predominant 10-kb transcript in all cells and organs tested except lung, liver, and kidney. Minor bands of about 5.5kb were detected in testis and placenta. Epitope-tagged SEP1 was recovered in the cytoplasmic fraction of transfected 293 cells and showed an apparent molecular mass of about 208kD. Fluorescence-tagged SEP1 localized to the cytoplasm, particularly the nuclear periphery, of transfected HeLa cells. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice