369649
TOPBP1 (Topoisomerase II Binding Protein 1, TOP2BP1, DNA Topoisomerase II-beta-binding Protein 1)
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£626.00
- SKU:
- 369649
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- TOPBP1 (Met1~Lys250)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- TOPBP1 is a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.The deduced 1522aa protein has a calculated molecular mass of about 171kD. TOPBP1 contains 8 repeating regions throughout its sequence that share similarity with repeat regions 1 and 2 of Rad4. It also has an auto-ADP-ribosylation site and 2 C-terminal nuclear localization signals. The 3-prime untranslated region contains 12 AUUUA mRNA-instability motifs. Northern blot analysis detected ubiquitous expression, with smearing characteristic of unstable mRNA. Transcripts above 10kb were detected only in heart. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice