PDE5A (Phosphodiesterase 5A, cGMP Specific, CGB-PDE, cGMP-binding cGMP-specific Phosphodiesterase, cGMP-specific 3',5'-cyclic Phosphodiesterase)

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£626.00

SKU:: 369605
Anwendung:: WB
Reinheit:: Purified by Protein A/G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Weitere Details:: PDE5A encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. The predicted 875aa human protein is approximately 96% identical to bovine PDE5A. Like the bovine protein, human PDE5A contains a cGMP-binding domain in its N-terminal portion and a catalytic domain in its C-terminal region. Recombinant PDE5A protein hydrolyzed cGMP in vitro. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.