ERI1 (Exoribonuclease 1, THEX1, 3'HEXO, 3'-5' exonuclease ERI1, Histone mRNA 3'-end-specific exoribonuclease, Enhanced RNAi Three Prime mRNA Exonuclease Homolog 1)

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£626.00

SKU:: 369517
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: ERI1 (Glu2~Lys249)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Metazoan replication-dependent histone mRNAs end in a highly conserved stem-loop structure that is required for regulation of histone mRNA stability and is bound by stem loop-binding protein (SLBP). Using the histone mRNA stem-loop sequence in RNA affinity purification assays, Dominski et al. (2003) isolated HEXO (histone mRNA end-specific exonuclease) protein from HeLa whole-cell extracts. They sequenced regions of HEXO protein using mass spectrometry and matched this protein to a full-length protein from a sequence database. HEXO encodes a 50kD protein containing a SAP domain capable of recognizing AT-rich scaffold-attachment regions (SARs) in chromosomal DNA, and a exonuclease domain similar to those of DEDD exonuclease family members. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice