369466
Bax (Bcl2 Associated X Protein, BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4)
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£524.00
- SKU:
- 369466
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1~Gly192 of human Bcl2 Associated X Protein expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Bax (Bcl-2 associated x protein) is an apoptosis regulating protein of the Bcl-2 family. The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stimulus. Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology. Bax is a 21kD protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.05-2ug/ml Western Blot: 0.05-2µg/ml Immunohistochemistry (frozen): 5-20ug/ml Immunohistochemistry (paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice