368502
ADAM17 (TACE, TNF-alpha-converting Enzyme, Disintegrin and Metalloproteinase Domain-containing Protein 17, CD156b)
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£544.00
- SKU:
- 368502
- Reinheit:
- Purified by Protein G affinity chromatography. Endotoxin: ≤0.001EU/ug
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Human
- Immunogen:
- Recombinant protein corresponding to human ADAM17 (TACE) ectodomain tagged to biotin, expressed in E. coli.
- Klon:
- D1(A12)
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Weitere Details:
- ADAM17 (Disintegrin and metalloproteinase domain-containing protein 17), also called TACE (Tumor necrosis factor-alpha-converting enzyme) is the prototype of the ADAM family of ectodomain shedding proteases (sheddase). ADAM17 is understood to be involved in the processing of TNF-alpha at the surface of the cell and from within the intracellular membranes of the trans-Golgi network. This process involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-alpha) and is of known physiological importance. ADAM17 is responsible for the processing of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands and enzymes, including cleaving epidermal growth factor receptor (EGFR) ligands and extracellular Notch1. The proteolytic cleavage is an indispensable activation event for many of these substrates, ADAM17 has emerged as an attractive therapeutic target for the treatment of inflammatory diseases (e.g. rheumatoid arthritis) or inflammation associated cancer. This cross-domain human antibody is a selective ADAM17 (TACE) antagonist and provides a unique alternative to small molecule metalloprotease inhibition. ADAM multidomain topology was exploited by first isolating an inhibitory human antibody (D1) that bound ADAM17-specific non-catalytic regions exclusively through its variable heavy (VH) domain. A D1-VH biased scFv phage-display library was then used to selectively isolate a new variable light (VL) chain that could simultaneously bind to the ADAM17 catalytic domain. Applications: Suitable for use in Blocking. Other applications not tested. Recommended Dilution: Blocking: Inhibits ADAM17 activity at 30ug/ml (200nM) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice