367401
Desmoglein 1
Größe
£850.00
- SKU:
- 367401
- Reinheit:
- Supernatant
- Lagerbedingungen:
- 4°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human polypeptide Desmoglein 1, extracellular part EII, EIII and EIV.
- Klon:
- Dsg1-P23
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Weitere Details:
- The desmogleins are a family of cadherins consisting of proteins DSG1, DSG2, DSG3, and DSG4. They play a role in the formation of desmosomes that join cells to one another. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160kD polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): ready-to-use (preincubation with 0.001% trypsin, addition of 2% milk powder to the incubation media, and microwave treatment is recommended) Immunohistochemistry (frozen): ready-to-use (treatment with 0.05 - 0.02 % Triton X-100 is recommended) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Versandbedingungen:
- Blue Ice