CD20 (MS4A1, B-Lymphocyte Antigen CD20, B1, Bp35, CD20 Antigen, CD20 Receptor, LEU- 16, CVID5) (Biotin)

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£341.00

SKU:: 367053
Anwendung:: Flow Cytometry
Reinheit:: Highly Purified
Lagerbedingungen:: 4°C Do Not Freeze
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Human tonsillar B cells
Klon:: 2H7
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Weitere Details:: CD20, a 33-36kD non-glycosylated type 1 transmembrane protein, is expressed by developing, resting and mature B cells, some follicular dendritic cells, and a small subset of mature T cells. B cell CD20 expression is lost upon differentiation into plasma cells. Functionally, CD20 activation contributes to B cell activation, proliferation, and differentiation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume or 0.1ml whole blood followed by a secondary staining with any fluorescent conjugated streptavidin Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.