367034
CD16 (Fc gamma RIII, NK cells)
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£358.00
- SKU:
- 367034
- Anwendung:
- Flow Cytometry
- Reinheit:
- Highly Purified
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human peripheral blood T lymphocytes
- Klon:
- 3G8
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Weitere Details:
- CD16 binds to the 50-65kD transmembrane protein of IgG FcgammaRIII, an surface antigen commonly associated with human NK cells. Besides surface expression on NK cells, it is also expressed on activated monocytes, macrophages, on neutrophils and placental trophoblasts in humans. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). CD16 is also useful in experiments related to immunohistochemical staining, immunoprecipitation, NK cell proliferation, blocking of phagocytosis and blocking of immunoglobulin binding through FcgammaRIII. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume followed by a secondary florescent conjugated anti-mouse antibody Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice