MRP2 (Canalicular Multispecific Organic Anion Transporter 1, ATP-binding Cassette Sub-family C Member 2, Canalicular Multidrug Resistance Protein, cMRP, Multidrug Resistance-associated Protein 2)

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£761.00

SKU:: 366971
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: GST-tagged recombinant fragment corresponding to the third cytoplasmic domain of human MRP2.
Klon:: 4C9.2
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Canalicular multispecific organic anion transporter 1 (UniProt Q92887; also known as ATP-binding cassette sub-family C member 2, Canalicular multidrug resistance protein, cMRP, Multidrug resistance-associated protein 2) is encoded by the ABCC2 (also known as CMOAT, CMOAT1, CMRP, DJS, MRP2) gene (Gene ID 1244) in human. MRP2/ABCC2 belongs to the family of human ATP-binding cassette (ABC) transporters that are classified into seven subfamilies from ABC-A to ABC-G based on their sequence similarities. P-glycoprotein (P-gp/ABCB1), MRP2/ABCC2, and breast cancer resistance protein (BCRP/ABCG2) are well known plasma membrane ABC transporters that mediate the export of intracellular drugs, drug conjugates and metabolites. MRP2/ABCC2 is the second member of the ABCC subfamily of 13 transporters (ABCC1 to ABCC13). MRP2 is expressed in the apical hepatocyte plasma membrane, renal proximal tubules and small intestine, where it plays a role in the elimination and oral bioavailability of drugs, xenotoxins and their metabolites. MRP2 primarily functions as an organic anion transporter to mediate the elimination of bile acids, GSH and conjugated metabolites of numerous drugs and other xenobiotics. Weakly basic drugs appear to be co-transported with GSH by MRP2. Methotrexate, anthracyclines (doxorubicin, epirubicin), mitoxantrone, cisplatin, and etoposide are well known chemotherapeutic substrates of MRP2. MRP2 contains 17-transmembrane helices (aa28-48, 69-89, 94-114, 127-147, 166-186, 314-334, 361-381, 438-458, 462-482, 545-565, 588-608, 972-992, 1034-1054, 1098-1118, 1120-1140, 1212-1232, 1235-1255), having its N-terminal end (aa1-27) exposed extracellularly and C-terminal end (aa1256-1545) at the cytoplasmic side. Two conserved nucleotide-binding domains (NBDs; aa671-678 and 1334-1341) mediate the binding and hydrolyzing ATP via an ATPase, thereby providing energy for substrates export from the cytoplasm to the extracellular space. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:50 detects MRP2 in human liver tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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