Lgr5 (GPR49, Leucine-rich Repeat-containing G-Protein Coupled Receptor 5, G-Protein coupled Receptor 49, G-Protein Coupled Receptor 67, G-Protein Coupled Receptor HG38, Orphan G Protein-Coupled Receptor HG38)

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£761.00

SKU:: 366968
Anwendung:: WB
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: GST-tagged recombinant human Lgr5/GPR49 fragment corresponding to leucine-rich repeats (LRRs) 7-11 within the N-terminal extracellular domain.
Klon:: 5G10.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Leucine-rich repeat-containing G-protein coupled receptor 5 (UniProt O75473; also known as G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Orphan G protein-coupled receptor HG38) is encoded by the LGR5 (also known as GPR49, GPR67, GRP49, HG38) gene (Gene ID 8549) in human. Lgr5 is a 7-transmembrane protein related to the receptors for the follicle stimulating hormone (FSH), the luteinizing hormone (LH), and the thyroid-stimulating hormone (TSH). Lgr5 is a Wnt signaling pathway targeted gene expressed in proliferative stem cells in various tissues, including small intestine, colon, stomach, and the hair follicle. The R-spondin (RSPO) family of secreted Wnt signaling agonists are known to bind Lgr5 and the related Lgr4 and Lgr6, and RSPO1 depends on its association wth Lgr4, Lgr5, or Lgr6 for its Wnt signaling enhancing effect. Human Lgr5 is synthesized with a propeptide sequence (aa1-21), the removal of which yields the mature 7-transmembrane (aa562-582 , 594-614, 639-659, 683-703, 723-743, 768-788, 803-823) G-protein-coupled receptor (GPCR), having its N-terminal end (aa22-561) at the extracellular side and its C-terminal end (aa824-907) at the cytoplasmic side. The N-terminal extracellular region contains a leucine-rich repeat (LRR) N-terminal (LRRNT) domain (aa25-66), followed by 16 LRRs (LRR 1 through LLR 16; aa67-446). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detects Lgr5 (GPR49) in 10ug of HEK293, HeLa, Jurkat, MCF-7, and Raji membrane extracts. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice