366955
Histone H3, dimethyl (Lys27) (H3K27me2, H3.3B, Lys27 Dimethylated, H3 histone, Family 3A, Lys27 Dimethylated, Family 3B, Lys27 Dimethylated, Lys27 Dimethylated)
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£869.00
- SKU:
- 366955
- Reinheit:
- Purified by affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- Peptide corresponding to aa22-32 from human Histone H3 (Lys27), KLH conjugated.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Weitere Details:
- Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. Applications: Suitable for use in Western Blot, ChIP and Dot Blot. Other applications not tested. Recommended Dilution: Dot Blot: 1:500, specifically detects histone H3 peptide with K27me2 modification, but not H3 peptides with unmodified K27 and other K27 modifications, H2A, H2B, or H4 peptides by dot blot analysis using AbSurance H2A, H2B, H4 Antibody Specificity Array Chromatin Immunoprecipitation: 12ul detects Lys27-dimethylated histone H3 occupancy at the -globin promoter region using HeLa nuclear extract Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice