Geminin

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£800.00

SKU:: 366952
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein fragment corresponding to human Geminin fused to GST-Tag.
Klon:: 4B10.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Geminin (UniProt O75496) is encoded by the GMNN gene (Gene ID 51053) in human. Geminin is a negative regulator of DNA-replication and the founding member of the Geminin family, whose members also include Idas (multicilin; McIdas) and GemC1 (Geminin coiled-coil domain-containing protein 1). Geminin binds Cdt1 and inhibits the mini-chromosome maintenance complex (MCM) loading onto chromatin and pre-replication complex (preRC) formation. Geminin is a 209aa protein that can form homodimer or heterodimers with either Idas or GemC1 via the middle coiled coil structure (aa94-144 of Geminin) in a head-to-tail fashion. The same region in Geminin (aa82-161) is also essential for Cdt1 interaction. A homeodomain-binding domain is located at the C-terminal region of Geminin (aa170-190). GemC1 is also implicated in DNA replication but at a later stage than Geminin. All three Geminin family members play important roles in cell proliferation and differentiation. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:250 detects nuclear geminin immunoreactivity in HUVECs, A431, and HeLa cells. Western Blot: 1:2000 detected geminin in 10ug of HEK293 cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice