CDK12 (Cyclin-dependent Kinase 12, CDC2-related Protein Kinase 7, Cdc2-related Kinase, Arginine/Serine-rich, Cell Division Cycle 2-related Protein Kinase 7, Cell Division Protein Kinase 12, CrkRS, hCDK12)

Größe

£761.00

SKU:: 366940
Reinheit:: Purified by affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to an N-terminal sequence of human CDK12. Species Sequence Homology: mouse and rat.
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Cyclin-dependent kinase 12 (EC 2.7.11.22; EC 2.7.11.23; UniProt Q9NYV4; also known as CDC2-related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, Cell division cycle 2-related protein kinase 7, Cell division protein kinase 12, CrkRS, hCDK12) is encoded by the CDK12 (also known as CRK7, CRKRS, KIAA0904) gene (Gene ID 51755) in human. CDK12 belongs to the CMGC Ser/Thr protein kinase subfamily of cyclin-dependent kinases (CDKs). Originally identified and characterized as an RNA Polymerase II C-terminal domain (CTD) kinase, CDK12 is involved in the 3′ end processing of the MYC and c-FOS mRNA transcripts. In addition, CDK12/CyclinK complex is implicated in the maintenance of genomic stability through the regulation of DNA damage response genes and as a determinant of PARP1/2 inhibitor sensitivity in cancer treatment. Human CDK12 is uncharacteristically large with a molecular weight of ∼160kD (most CDKs are about 50kD) and contains large N- and C-terminal extensions flanking the kinase domain (aa727-1020). These extensions contain multiple stretches of low complexity sequence and RS domains characteristic of splicing factors and regulators believed to mediate protein-protein interactions. As expected of RS domain-containing proteins, CDK12 is found in nuclear speckles representing storage sites for splicing factors. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml detects CDK12 in 45ug of HeLa nuclear extract Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice