Cdh1, NT (Fizzy-related Protein Homolog, CDC20-like Protein 1, CDH1alpha, CDH1beta, Cdh1/Hct1 Homolog, hCDH1, Fzr, Fzr1, Fzr2)

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£761.00

SKU:: 366939
Anwendung:: WB
Reinheit:: Purified by Protein G affinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Mouse
Immunogen:: GST-tagged recombinant protein corresponding to human Cdh1, N-terminal fragment. Species sequence homology: rat
Klon:: 15A9.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Weitere Details:: Fizzy-related protein homolog (UniProt Q9UM11; also known as CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1, Fzr) is encoded by the FZR1 (also known as CDH1, FYR, FZR, KIAA1242) gene (Gene ID 51343) in human. The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase involved in cell cycle regulation. APC/C is activated upon sequential binding to the Cdc20 and Cdh1 adaptor proteins. Cdc20 associates with APC/C during early mitosis and principally regulates mitotic progression, while Cdh1 interacts with the APC/C from late mitosis onwards until the following G1/S transition. DNA-end resection factor CtIP. Cdk-mediated Cdh1 phosphorylation prevents Cdh1 from associating with APC/C prematurely during S/G2 phase, while Cdc14B phosphatase-dependent APC/C-Cdh1 activation in response to DNA damage during G2 phase leads to Polo-like kinase 1 (Plk1) degradation. Following DNA double-strand breaks (DSB) repair, Cdk1 and Plk1 are reactivated to allow cell cycle progression from G2 into mitosis. Cdh1-depleted cells or Cdh1 / mice exhibit elevated levels of DNA damage and chromosomal aberrations. APC/C-Cdh1 interacts with the KEN box motif on its target proteins and known APC/C-Cdh1 substrates include the the de-SUMOylation enzyme SENP2, and and the fragile X syndrome protein FMRP. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 detects Cdh1 in 10 ug of embryonic (E13-14 & E16) and neonatal (P1 & P7) mouse brain tissue lysates, as well as in mouse embryonic stem cell (mESC) and neural stem cell (mNSC) lysates. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice