BCL11B (CTIP2, B-Cell Lymphoma/Leukemia 11B, B-Cell CLL/Lymphoma 11B, mRit1, BCL-11B, COUP-TF-interacting Protein 2, Radiation-induced Tumor Suppressor Gene 1 Protein)

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£809.00

SKU:: 366926
Reinheit:: Purified by Protein G affinity chromatography
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rat
Immunogen:: Full-length recombinant protein corresponding to mouse CTIP2/BCL11B
Klon:: 25B6
Format:: Supplied as a liquid in 15mM HEPES, 150mM sodium chloride, pH 7.5, 0.02% sodium azide
Weitere Details:: B-cell lymphoma/leukemia 11B (UniProt Q99PV8; also known as B-cell CLL/lymphoma 11B, BCL-11B, COUP-TF-interacting protein 2, mRit1, Radiation-induced tumor suppressor gene 1 protein) is encoded by the Bcl11b (also known as Ctip2, Rit1) gene (Gene ID 58208) in murine species. CTIP2/CBL11B is a C2H2 zinc finger protein that binds GC-rich response elements and is reported to associate with a variety of cofactors, including the corepressor complexes NuRD, Sirt1, histone acetyltransferase (HAT) p300, functioning both as a transcriptional repressor and activator. CTIP2/CBL11B is required at multiple developmental checkpoints for T cell commitment and maintenance. CTIP2/CBL11B expression is initiated at double-negative 2 (DN2) stage of T cell development and is maintained in mature T lymphocytes, with lower levels found in NK cells, but not in B cells, myeloid or dendritic cells. Bcl11b-/- mice die shortly after birth due to a blockade of thymocyte development at the DN2 stage. Bcl11b-deficient DN2 thymocytes are highly proliferative and retain the potential to differentiate into NK cells and myeloid lineages, but not mature T-cells. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml using 10ug of HEK293 cell lysate Immunohistochemistry (Paraffin): 1:240 detects CTIP2/BCL11B in mouse brain tissue sections. Heat-induced epitope retrieval (HIER) is recommended Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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