365597
WNK1 (WNK Lysine Deficient Protein Kinase 1, HSN2, KDP, PHA2C, PRKWNK1, HSAN2, Protein Kinase,Lysine Deficient 1, Hereditary Sensory Neuropathy, Type II, Erythrocyte 65kD protein, Kinase deficient protein)
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£626.00
- SKU:
- 365597
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Lys1954-Ala2231 of human WNK1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- The WNK1 protein is composed of 2382aa. The protein contains a small N-terminal domain followed by the kinase domain and a long C-terminal tail. The kinase domain has some similarity to the MEKK protein kinase family. The protein appears to be part of the ERK5 MAP kinase pathway upstream of MEKK2 / MEKK3 and to function as a tetramer. It selectively binds to and phosphorylates synaptotagmin 2 (SYT2) within its calcium binding C2 domains. It activates the serum- and glucocorticoid-inducible protein kinase SGK1, leading to activation of the epithelial sodium channel. It along with WNK4 stimulates clathrin-dependent endocytosis of renal outer medullar potassium 1 (ROMK1). It (and WNK4) interactes with intersectin (ITSN1, ITSN2). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KVGRFSV SKTEDKITDT KKEGPVASPP FMDLEQAVLP AVIPKKEKPE LSEPSHLNGP SSDPEAAFLS RDVDDGSGSP HSPHQLSSKS LPSQNLSQSL SNSFNSSYMS SDNESDIEDE DLKLELRRLR DKHLKEIQDL QSRQKHEIES LYTKLGKVPP AVIIPPAAPL SGRRRRPTKS KGSKSSRSSS LGNKSPQLSG NLSGQSAASV LHPQQTLHPP GNIPESGQNQ LLQPLKPSPS SDNLYSAFTS DGAISVPSLS APGQGTSSTN TVGATVNSQA A Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice