365434
TREH (Trehalase, TRE, TREA, Brush Border Membrane Glycoprotein, Alpha,Alpha-Trehalase, Alpha,Alpha-Trehalose Glucohydrolase)
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£537.00
- SKU:
- 365434
- Anwendung:
- WB
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa151-411 of mouse TREH, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Weitere Details:
- Trehalose (1-alpha-D-glucopyranosyl alpha-D-glucopyranoside) is widely distributed in nature, being the storage disaccharide of fungi and the blood sugar of insects. Trehalase (EC 3.2.1.28) is an intrinsic glycoprotein of the small intestine and renal brush border membranes that hydrolyzes trehalose to 2 glucose molecules. The deduced 583aa protein has a calculated molecular mass of 66.6kD. It has an N-terminal signal peptide, 5 potential N-glycosylation sites, and a C-terminal hydrophobic region for glycosylphosphatidylinositol (GPI) attachment. Northern blot analysis detected a 2.0-kb transcript mainly in kidney, liver, and small intestine.The trehalase signature sequence from aa162 to 175 of mouse Treh shares 100% identity with the corresponding region in human TREH. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SLIYSKHPFI VPGGRFVEFY YWDSYWVMEG LLLSEMASTV KGMLQNFLDL VKTYGHIPNG GRIYYLQRSQ PPLLTLMMDR YVAHTKDVAF LQENIGTLAS ELDFWTVNRT VSVVSGGQSY VLNRYYVPYG GPRPESYRKD AELANSVPEG DRETLWAELK AGAESGWDFS SRWLVGGPDP DLLSSIRTSK MVPADLNAFL CQAEELMSNF YSRLGNDTEA TKYRNLRAQR LAAMEAVLWD EQKGAWFDYD LEKGKKNLEF Y Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice