TIEG1 (TGF Beta Inducible Early Response Gene 1, KLF10, EGRA, TIEG, Krueppel-Like Factor 10, TGFB Inducible Early Growth Response, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1)

Größe

£482.00

SKU:: 365305
Reinheit:: Purified by immunoaffinity chromatography.
Lagerbedingungen:: -20°C
Hersteller:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Ser210 of human TIEG1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:: Tachibana et al. characterized the expression and function in exocrine pancreatic epithelial cells of the TGF-beta-inducible early gene (TIEG), a Kruppel-like zinc finger transcription factor- encoding gene previously isolated from mesodermally derived osteoblastic cells by Subramaniam et al. Tachibana et al. demonstrated that the gene is expressed in both acinar and ductular epithelial cell populations from the exocrine pancreas. In addition, they showed that the expression of TIEG is regulated by TGF-beta-1 (TGFB1) as an early response gene in pancreatic epithelial cell lines. Overexpression of TIEG in a TGF-beta-sensitive epithelial cell line was sufficient to induce apoptosis. Thus, Tachibana et al. concluded that TIEG plays a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MLNFGASLQQ TAEERMEMIS ERPKESMYSW NKTAEKSDFE AVEALMSMSC SWKSDFKKYV ENRPVTPVSD LSEEENLLPG TPDFHTIPAF CLTPPYSPSD FEPSQVSNLM APAPSTVHFK SLSDTAKPHI AAPFKEEEKS PVSAPKLPKA QATSVIRHTA DAQLCNHQTC PMKAASILNY QNNSFRRRTH LNVEAARKNI PCAAVSPNRS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Versandbedingungen:: Blue Ice