365234
TBK1 (TANK Binding Kinase 1, NAK, T2K, NF-kappa-B-activating kinase, Serine/threonine-protein kinase TBK1)
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£626.00
- SKU:
- 365234
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Trp9-Val310 of human TBK1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. TANK-Binding Kinase 1 is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. The cDNA encodes a deduced 730aa protein that contains a leucine zipper and a helix-loop-helix motif within its C-terminal half. Unlike IKBKA and IKBKB, which each contain 2 serines in their respective activation loops, NAK contains a glutamic acid (glu168) and a serine, which must be phosphorylated for activation. Northern blot analysis revealed ubiquitous expression of a 2.2-kb transcript, with highest expression in testis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Versandbedingungen:
- Blue Ice