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365150

SPC (Surfactant Associated Protein C, SFTPC, PSP-C, SFTP2, SP-C, SP5, Pulmonary Surfactant Protein C, Pulmonary surfactant-associated proteolipid SPL Val)

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£267.00

SKU:
365150
Reinheit:
Purified by Protein A and peptide affinity chromatography.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to Phe95-Ile194 of rat SPC, expressed in E. coli.
Format:
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:
SP-C is a fragment of a much larger precursor protein of 21kD. The precursor contains an exceedingly hydrophobic region of 34aa that comprises most of the mature SP C, and a unique poly-valine domain.Pulmonary surfactant-associated protein C is a membrane protein which manufactures surfactant. Humans and animals born lacking SP-C tend to develop progressive interstitial pneumonitis. The SFTPC gene encodes pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. It is produced exclusively by type II alveolar epithelial cells in the lung. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Positive Control Included: 365150A: SPC, Positive Control Supplied as a recombinant protein SPC in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Versandbedingungen:
Blue Ice