365147
SPARCL1 (SPARC Like Protein 1, SC1, PIG33, Mast9, Hevin, High endothelial venule protein)
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£438.00
- SKU:
- 365147
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gly298-His545 of human SPARCL1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Hevin is an evolutionarily conserved, 664aa secreted protein. Hevin contains 7 potential N-linked glycosylation sites, an N-terminal signal peptide, a long acidic region rich in glu and asp, a cysteine-rich domain, and a C-terminal region with a 12-residue segment homologous to the calcium-binding loops of EF-hand structures in the calmodulin family of proteins. The 232 C-terminal amino acids of Hevin are 62% identical to the homologous portion of SPARC. Northern blot analysis revealed high expression of a 2.7-kb Hevin transcript in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. Unlike SPARC, Hevin is absent from flat umbilical vein endothelial cells. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice