365128
SOD4 (Superoxide Dismutase, Copper Chaperone, CCS, Copper Chaperone For Superoxide Dismutase)
Größe
£482.00
- SKU:
- 365128
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gln23-Gln269 of human SOD4, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Copper (Cu) is required for aerobic life and yet, paradoxically, is highly toxic. This apparent contradiction has been rationalized by assuming that Cu, like other redox-active metals, is sequestered in nonreactive forms as it is transported into cells and moves through cellular compartments. Culotta et al. (1997) determined that one such Cu chaperone protein, Lys7, specifically delivers Cu to copper/zinc superoxide dismutase (Sod1) in S. cerevisiae. By searching EST databases, they identified cDNAs encoding the human Lys7 homolog, which they named CCS (copper chaperone for SOD1). The predicted 274aa human protein is 28% identical to Lys7. CCS complemented a yeast Lys7 mutation, demonstrating that CCS is a functional homolog of Lys7. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice