365046
SHH (Hedgehog Homolog, Sonic, HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N)
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£524.00
- SKU:
- 365046
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Pro36-Ala193 of human SHH, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- Sonic hedgehog (Shh) is a member of a small family of secreted proteins that are essential for development in both vertebrates and invertebrates. In mammals, there are three related hedgehog genes (sonic; desert; Indian) which share approximately 60aa identity. Human Shh is synthesized as a 437aa precursor that contains a 24aa signal sequence and a 413aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20kD, 174aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34kD, 239aa C-terminal fragment. The 20kD Shh-N fragment is the core of the active hedgehog molecule. It begins with a Cys and ends with a Gly, and both amino acids are sites of lipid modification. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice