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365039

SH3BGRL (SH3 Domain Binding Glutamic Acid Rich Protein Like Protein)

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£517.00

SKU:
365039
Reinheit:
Purified by immunoaffinity chromatography.
Lagerbedingungen:
-20°C
Hersteller:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to Met1-Ala114 of human SH3BGRL, expressed in E. coli.
Format:
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Weitere Details:
Proline-rich peptide sequences have been shown to play important roles in protein-protein interactions that occur in signal transduction pathways. Specific proline-rich consensus sequences that bind SH3 domains have been identified. The predicted 114aa SH3BGRL protein has a potential cell attachment sequence. A proline-rich sequence containing an SH3-binding motif is present in the middle of the protein. The SH3BGRL protein shares 60% identity with the proline-rich central region of SH3BGR. Northern blot analysis detected a single SH3BGRL transcript of approximately 1.9 kb that was present in all tissues examined. Mouse cDNA encoding a deduced protein that is 95% identical to human SH3BGRL. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Versandbedingungen:
Blue Ice