364706
POFUT1(Protein O-Fucosyltransferase 1, O-FUT, O-Fuc-T, FUT12, GDP-Fucose Protein O-Fucosyltransferase 1, Peptide-O-fucosyltransferase 1)
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£537.00
- SKU:
- 364706
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to His243-Phe393 of mouse POFUT1, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Weitere Details:
- -fucosyltransferase 1 (POFUT1) is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues between the second and third conserved cysteines in EGF-like repeats on the Notch protein. The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-Beta-L-fucose as a donor substrate and transfers the fucose in O linkage to the protein producing fucose-alpha-O-serine/threonine. When the gene for POFUT1 is knocked out, or the expression is decreased to very low levels, all Notch signaling is destroyed, which means that fucose on Notch is absolutely essential for Notch function. Why this is the case is not yet well understood. Almost all glycosyltransferases reside in the Golgi apparatus. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice