364689
PLOD1 (Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1, LLH, PLOD, LH1, Lysine Hydroxylase,Ehlers-Danlos Syndrome Type VI, Lysyl hydroxylase 1)
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£626.00
- SKU:
- 364689
- Reinheit:
- Purified by immunoaffinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gln636-Pro727 of human PLOD1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Weitere Details:
- PLOD1 encode a polypeptide of 709aa residues and a signal peptide of 18aa. The human coding sequence shows 72% identity to the chick sequences at the nucleotide level and 76% identity at the aa level. The C-terminal region is especially well conserved; residues 588 to 727 show 94% identity between the 2 species and residues 639 to 715 show 99% identity. The gene for lysyl hydroxylase, symbolized PLOD (for procollagen-lysine, 2-oxoglutarate 5-dioxygenase), was mapped to chromosome 1 by Southern blot analysis of human/mouse somatic cell hybrids, to the 1pter-1p34 region by use of cell hybrids that contained various rearrangements of human chromosome 1, and to 1p36.3-p36.2 by in situ hybridization. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice