364658
PKD1 (Protein Kinase D1, PKC-MU, PKCM, PK-D1, PRKCM, Protein Kinase C,Mu)
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£479.00
- SKU:
- 364658
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Thr651-Gln876 human PKD1, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Weitere Details:
- By screening a placenta cDNA library using a PCR fragment derived from the second conserved domain within the catalytic site of ser/thr protein kinases, Johannes et al. obtained a full-length cDNA encoding PKC-mu. The deduced 912aa PRKCM protein has a calculated molecular mass of 102kD. PRKCM has 2 characteristic cysteine-rich repeats, which are conserved among nearly all members of the PKC family, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity. Northern blot analysis detected a single PRKCM transcript of 3.8 kb at low, constitutive levels in all tissues examined. The authors verified PRKCM kinase activity by transfection of a PRKCM expression vector and detecting changes in protein phosphorylation patterns by Western blot analysis. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice