364639
PIK3AP1 (Phosphoinositide-3-Kinase Adaptor Protein 1, BCAP, B-cell adapter for phosphoinositide 3-kinase, B-cell phosphoinositide 3-kinase adapter protein 1)
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£626.00
- SKU:
- 364639
- Reinheit:
- Purified by Protein A and peptide affinity chromatography.
- Lagerbedingungen:
- -20°C
- Hersteller:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Leu489-Ser730 of human PIK3AP1, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Weitere Details:
- Using an affinity purification strategy with the N-terminal SH2 domain of the phosphoinositide 3-kinase (PI3K) p85 subunit and anti-phosphotyrosine antibody, followed by screening a chicken DT40 B-cell cDNA library and a mouse spleen cDNA library, Okada et al. (2000) isolated cDNAs encoding chicken and mouse Pik3ap1, which they called Bcap. They also identified Bcap splice variants. Northern blot analysis of mouse tissues detected expression predominantly in spleen, with lower levels in thymus, liver, and lung. Expression was also detected in mouse macrophage and B-cell lines, but not in T-cell, plasma cell, or mast cell lines.Using mouse and chicken B- cell lines, Okada et al. (2000) determined that Bcap is a protein tyrosine kinase substrate that couples B-cell receptor to PI3K activation. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LE GNSMGMTNLE RDQCHLGQEE DVYHTVDDDE AFSVDLASRP PVPVPRPETT APGAHQLPDN EPYIFKVFAE KSQERPGNFY VSSESIRKGP PVRPWRDRPQ SSIYDPFAGM KTPGQRQLIT LQEQVKLGIV NVDEAVLHFK EWQLNQKKRS ESFRFQQENL KRLRDSITRR QREKQKSGKQ TDLEITVPIR HSQHLPAKVE FGVYESGPRK SVIPPRTELR RGDWKTDSTS STASSTSNRS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Versandbedingungen:
- Blue Ice